Visualisation of electrochemical processes by coupled electrochemistry and fluorescence microscopy

Release monitoring of single cells on a microfluidic device coupled with fluorescence microscopy and electrochemistry.

A method for monitoring the biological exocytotic phenomena on a microfluidic system was proposed. A microfluidic device coupled with functionalities of fluorescence imaging and amperometric detection has been developed to enable the real-time monitoring of the exocytotic events. Exocytotic release of single SH-SY5Y neuroblastoma cells was studied. By staining the cells located on integrated mi...

Membrane Electrochemistry: Electrochemical Processes in Bilayer Lipid Membrane

Observation of nanometer-sized electro-active defects in insulating layers by fluorescence microscopy and electrochemistry.

We report a method to study electro-active defects in passivated electrodes. This method couples fluorescence microscopy and electrochemistry to localize and size electro-active defects. The method was validated by comparison with a scanning probe technique, scanning electrochemical microscopy. We used our method for studying electro-active defects in thin TiO2 layers electrodeposited on 25 μm ...

Imaging of podocyte foot processes by fluorescence microscopy.

Visualizing podocyte foot processes requires electron microscopy, a technique that depends on special equipment, requires immunogold for colabeling, and does not take advantage of the growing number of in vivo fluorophores available. To address these limitations, we developed a genetic strategy to allow detailed visualization of single podocytes and their foot processes by conventional fluoresc...

Fluorescence-Enabled Electrochemical Microscopy with Dihydroresorufin as a Fluorogenic Indicator

Recently, we introduced a new electrochemical imaging technique called fluorescence-enabled electrochemical microscopy (FFEM). The central idea of FEEM is that a closed bipolar electrode is utilized to electrically couple a redox reaction of interest to a complementary fluorogenic reaction converting an electrochemical signal into a fluorescent signal. This simple strategy enables one to use fl...